Guiochon-Mantel A, Lescop P, Christin-Maitre S, Loosfelt H, Perrot-Applanat M, Milgrom E
Hormones et Reproduction INSERM U 135, Faculté de Médecine Le Kremlin Bicêtre, France.
EMBO J. 1991 Dec;10(12):3851-9. doi: 10.1002/j.1460-2075.1991.tb04954.x.
The nuclear localization of the progesterone receptor is mediated by two signal sequences: one is constitutive and lies in the hinge region (between the DNA and steroid binding domains), the other is hormone dependent and is localized in the second zinc finger of the DNA binding domain. The use of various inhibitors of energy synthesis in cells expressing permanently or transiently the wild-type receptor or a receptor mutated within the nuclear localization signals, demonstrated that the nuclear residency of the receptor reflects a dynamic situation: the receptor diffusing into the cytoplasm and being constantly and actively transported back into the nucleus. The existence of this nucleo-cytoplasmic shuttle mechanism was confirmed by receptor transfer from one nucleus to the other in heterokaryons. Preliminary evidence was obtained, using oestrogen receptor, that this phenomenon may be of general significance for steroid receptors.
一个是组成型的，位于铰链区（在DNA和类固醇结合域之间），另一个是激素依赖性的，位于DNA结合域的第二个锌指中。在稳定或瞬时表达野生型受体或核定位信号内发生突变的受体的细胞中，使用各种能量合成抑制剂的实验表明，受体在核内的驻留反映了一种动态情况：受体扩散到细胞质中，并不断且主动地被转运回细胞核。通过异核体中受体从一个细胞核转移到另一个细胞核，证实了这种核质穿梭机制的存在。使用雌激素受体获得的初步证据表明，这种现象可能对类固醇受体具有普遍意义。
