Pukacka Stanisława, Ratajczak Ewelina, Kalemba Ewa
Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik, Poland.
J Plant Physiol. 2009 Sep 1;166(13):1381-90. doi: 10.1016/j.jplph.2009.02.013. Epub 2009 Apr 8.
Levels of sucrose and raffinose family oligosaccharides (RFOs) (raffinose and stachyose) were determined in beech (Fagus sylvatica L.) seeds during development, maturation, desiccation and storage. An increase in RFOs and a marked decrease in the S:(R+St) ratio (i.e. mass ratio of sucrose to the sum of RFOs) were observed at the time of desiccation tolerance (DT) acquisition by seeds. In seeds stored at -10 degrees C through 1, 4, 7, and 12 years, changes in sucrose, raffinose and stachyose levels and in alpha-galactosidase activity were noted. The S/R+St ratio and alpha-galactosidase activity significantly increased in seeds after 7 and 12 years of storage, when a marked decrease in viability, measured as germination capacity, was recorded. Germination capacity was found to be strongly correlated with sucrose content, the S:(R+St) ratio, and alpha-galactosidase activity. A strong positive correlation was found between germination capacity and stachyose content. The results clearly indicated that the composition of RFOs in beech seeds is closely related to DT acquisition and seed viability during storage.
在欧洲山毛榉（Fagus sylvatica L.）种子的发育、成熟、干燥和储存过程中，测定了蔗糖和棉子糖家族寡糖（RFOs，即棉子糖和水苏糖）的含量。在种子获得耐干燥性（DT）时，观察到RFOs含量增加，而S:(R+St) 比值（即蔗糖与RFOs总和的质量比）显著降低。在-10℃下储存1、4、7和12年的种子中，记录了蔗糖、棉子糖和水苏糖含量以及α-半乳糖苷酶活性的变化。在储存7年和12年后的种子中，S/R+St比值和α-半乳糖苷酶活性显著增加，此时以发芽能力衡量的活力显著下降。发现发芽能力与蔗糖含量、S:(R+St) 比值以及α-半乳糖苷酶活性密切相关。发芽能力与水苏糖含量之间存在强烈的正相关。结果清楚地表明，欧洲山毛榉种子中RFOs的组成与DT的获得以及储存期间种子活力密切相关。
