Zhang Xinhai, Li Jinhua, Wong Desmond K W, Wagner Thomas E, Wei Yanzhang
Oncology Research Institute of Greenville Hospital System University Medical Center, Greenville, SC 29605, USA.
Oncol Rep. 2009 May;21(5):1147-52.
Noni juice as a folk medicine has been used for over two thousand years. Recently, some active ingredients of Noni juice have been successfully isolated and intensively studied. Because dendritic cells (DCs) are central regulators both in priming innate and adaptive immune responses and in maintaining self tolerance, in the current study we treated DCs with fermented Noni Exudate (fNE) in order to explore their function in regulating other immune cells. It was shown that fNE-treated DCs stimulate proliferation of splenocytes, among which, B cells are the major responsive cell group. The proliferative response of B cells to fNE-treated DCs is cell contact-dependent, CD40L-independent; and the adhesion feature of DCs was enhanced to form large DC-B conjugation cluster. Moreover, it was demonstrated that fNE-treated DCs promote B cell differentiation and Ig class switching. These results lay a foundation for the further exploration of fNE as a biological response modifier in the immune system.
诺丽果汁作为一种民间药物已经使用了两千多年。最近，诺丽果汁的一些活性成分已被成功分离并深入研究。由于树突状细胞（DCs）在启动先天性和适应性免疫反应以及维持自身耐受性方面都是核心调节因子，因此在本研究中，我们用发酵诺丽渗出液（fNE）处理DCs，以探索它们在调节其他免疫细胞中的功能。结果表明，经fNE处理的DCs刺激脾细胞增殖，其中B细胞是主要的反应细胞群。B细胞对经fNE处理的DCs的增殖反应是细胞接触依赖性的，不依赖CD40L；并且DCs的粘附特性增强，形成大的DC-B结合簇。此外，还证明经fNE处理的DCs促进B细胞分化和Ig类别转换。这些结果为进一步探索fNE作为免疫系统中的生物反应调节剂奠定了基础。
