Ong Alan H K, Vellayan Subramaniam
Department of Bioscience and Chemistry, Universiti Tunku Abdul Rahman, Kuala Lumpur, Malaysia.
Zoo Biol. 2008 Jan;27(1):62-9. doi: 10.1002/zoo.20163.
The amplification of the highly conserved chromo-helicase-DNA binding region found in both the Z and W chromosome was evaluated with three sets of primers (P8/P2, 1237L/1272H and 2550F/2718R). DNA extracted from feathers through a simple boiling method was used to address its reliability in generating the sex-linked bands. All the bird samples, including the seven bird families that have not been reported previously, were successfully amplified with the primer set 2550F/2718R. The resulting polymerase chain reaction products showed clearly resolved fragments on a conventional agarose gel electrophoresis with size differences ranging from 80 to 540 bp between the two respective ZW gene copies. Although the P8/P2 primer was not as effective under the same conditions, it was able to produce well-resolved Z and W bands from bird species under the Antidea family, whereas the 2250F/2718R primer set only produced a single amplified fragment of a different size between the male and the female. Zoo Biol 27:62-69, 2008. (c) 2007 Wiley-Liss, Inc.
使用三组引物（P8/P2、1237L/1272H和2550F/2718R）评估了在Z和W染色体中均发现的高度保守的染色体解旋酶-DNA结合区域的扩增情况。通过简单煮沸法从羽毛中提取的DNA用于验证其在产生性别连锁条带方面的可靠性。所有鸟类样本，包括之前未报道过的七个鸟类家族，均使用引物组2550F/2718R成功扩增。所得的聚合酶链反应产物在传统琼脂糖凝胶电泳上显示出清晰分辨的片段，两个相应的ZW基因拷贝之间的大小差异在80至540 bp之间。尽管P8/P2引物在相同条件下效果不佳，但它能够从蚁鸟科的鸟类物种中产生清晰分辨的Z和W条带，而2250F/2718R引物组在雄性和雌性之间仅产生一个不同大小的单一扩增片段。《动物学杂志》27:62 - 69，2008年。（c）2007威利 - 利斯公司。
