Djaković-Sekulić Tatjana, Perisić-Janjić Nada, Djurendi Evgenija
Department of Chemistry, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia.
Biomed Chromatogr. 2009 Aug;23(8):881-7. doi: 10.1002/bmc.1200.
The chromatographic behaviour of salicylic acid derivatives was investigated using reversed-phase high performance thin-layer chromatography (RP HPTLC) with methanol-water and dioxane-water binary mixtures as mobile phase in order to establish relationships between chromatographic data and selected physico-chemical parameters that are related to ADME (absorption, distribution, metabolism and elimination). Some of the investigated compounds were screened for antioxidant activity. Examination of chromatographic behaviour revealed a linear correlation between R(M) values and the volume fraction of mobile phase modifier. Obtained R(M)(0) values were correlated with lipophilicity, solubility, human intestinal absorption, plasma-protein binding, and blood-brain barrier data. The comparison among chromatographic data obtained by two mobile phase was performed with a statistical technique, principle component analysis.
使用反相高效薄层色谱法（RP HPTLC），以甲醇 - 水和二氧六环 - 水二元混合物作为流动相，研究了水杨酸衍生物的色谱行为，以便建立色谱数据与与药物代谢动力学（吸收、分布、代谢和排泄）相关的选定物理化学参数之间的关系。对一些研究的化合物进行了抗氧化活性筛选。色谱行为研究表明，比移值（R(M)）与流动相改性剂的体积分数之间存在线性关系。获得的R(M)(0)值与亲脂性、溶解度、人体肠道吸收、血浆蛋白结合和血脑屏障数据相关。采用主成分分析这一统计技术对两种流动相获得的色谱数据进行了比较。
