采用紫外检测的毛细管区带电泳法及电喷雾电离四极杆/飞行时间质谱联用检测法分析奶粉中的三聚氰胺。

Analysis of melamine in milk powder by CZE using UV detection and hyphenation with ESI quadrupole/TOF MS detection.

作者信息

Klampfl Christian W, Andersen Liisa, Haunschmidt Manuela, Himmelsbach Markus, Buchberger Wolfgang

机构信息

Institute of Analytical Chemistry, Johannes Kepler University, Linz, Austria.

出版信息

Electrophoresis. 2009 May;30(10):1743-6. doi: 10.1002/elps.200800786.

DOI:10.1002/elps.200800786

PMID:19360779

Abstract

An easy to employ method for the determination of melamine in milk powder samples using CZE with either UV or MS detection is presented. The separation capabilities of CZE allowed keeping sample preparation rather simple. Before analysis, milk powder samples were dissolved in water, de-fatted with dichloromethane and diluted with ACN to precipitate the protein, filtrated and injected without further pretreatment. The developed CZE method allowed the determination of melamine in milk powder down to a level of 0.50 mg/kg using UV detection and 0.25 mg/kg when ESI quadrupole/TOF MS was employed for detection.

摘要

本文介绍了一种使用毛细管区带电泳（CZE）结合紫外（UV）或质谱（MS）检测来测定奶粉样品中三聚氰胺的简便方法。CZE的分离能力使得样品制备相当简单。分析前，将奶粉样品溶于水，用二氯甲烷脱脂，并用乙腈稀释以沉淀蛋白质，过滤后无需进一步预处理即可进样。所建立的CZE方法采用紫外检测时可测定奶粉中低至0.50 mg/kg的三聚氰胺，采用电喷雾四极杆/飞行时间质谱（ESI quadrupole/TOF MS）检测时可测定低至0.25 mg/kg的三聚氰胺。