Li Wei, Zheng Yongping, Zhang Tingwei, Wu Songjie, Zhang Jue, Fang Jing
Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
College of Engineering, Peking University, Beijing 100871, China.
Sensors (Basel). 2018 Mar 9;18(3):828. doi: 10.3390/s18030828.
Molecularly imprinted polymer (MIP) films prepared by bulk polymerization suffer from numerous deficiencies, including poor mass transfer ability and difficulty in controlling reaction rate and film thickness, which usually result in poor repeatability. However, polymer film synthesized by electropolymerization methods benefit from high reproducibility, simplicity and rapidity of preparation. In the present study, an Au film served as the refractive index-sensitive metal film to couple with the light leaked out from optical fiber core and the electrode for electropolymerizing MIP film simultaneously. The manufactured probe exhibited satisfactory sensitivity and specificity. Furthermore, the surface morphology and functional groups of the synthesized MIP film were characterized by Atomic Force Microscopy (AFM) and Fourier transform infrared microspectroscopy (FTIR) for further insights into the adsorption and desorption processes. Given the low cost, label-free test, simple preparation process and fast response, this method has a potential application to monitor substances in complicated real samples for out-of-lab test in the future.
通过本体聚合法制备的分子印迹聚合物（MIP）膜存在诸多缺陷，包括传质能力差、难以控制反应速率和膜厚度，这通常导致重复性差。然而，通过电聚合法合成的聚合物膜具有制备过程的高重现性、简便性和快速性等优点。在本研究中，金膜用作折射率敏感金属膜，用于耦合从光纤纤芯泄漏的光，并同时作为电聚合MIP膜的电极。所制备的探针表现出令人满意的灵敏度和特异性。此外，通过原子力显微镜（AFM）和傅里叶变换红外显微光谱（FTIR）对合成的MIP膜的表面形态和官能团进行了表征，以进一步深入了解吸附和解吸过程。鉴于该方法成本低、无需标记检测、制备过程简单且响应快速，未来有可能应用于监测复杂实际样品中的物质，以进行实验室外测试。
