Ali M Monsur, Li Yingfu
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, ON L8N 3Z5, Canada.
Angew Chem Int Ed Engl. 2009;48(19):3512-5. doi: 10.1002/anie.200805966.
Target detection by the naked eye: The action of an RNA-cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single-stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex-binding dye, the color of the dye changed from blue to purple.
将响应配体结合的RNA切割变构DNA酶的作用与滚环扩增过程偶联，以生成用于比色传感的长单链DNA分子（见示意图）。在双链结合染料存在的情况下，所得DNA与互补PNA序列杂交后，染料颜色从蓝色变为紫色。
