Mouri Ryota, Oishi Tohru, Torikai Kohei, Ujihara Satoru, Matsumori Nobuaki, Murata Michio, Oshima Yasukatsu
Department of Chemistry, Osaka University, Machikaneyama, Toyonaka, Japan.
Bioorg Med Chem Lett. 2009 May 15;19(10):2824-8. doi: 10.1016/j.bmcl.2009.03.103. Epub 2009 Mar 26.
Ladder-shaped polyether (LSP) compounds represented by brevetoxins and ciguatoxins were largely discovered in association with seafood poisoning. Thus, a quick quantification method for LSPs is potentially important. We examined a surface plasmon resonance method using desulfated-yessotoxin (dsYTX) immobilized on a sensor chip and phosphodiesterase PDEII in a inhibition detection mode. Yessotoxin, brevetoxin B and synthetic LSP derivatives showed clear inhibition against PDEII binding to the immobilized dsYTX, by which their half inhibitory concentrations were successfully estimated. This inhibition method appeared to be superior in specificity to direct binding assays where binding proteins to LSP was immobilized on a sensor chip.
