Cazander G, van Veen K E B, Bernards A T, Jukema G N
Department of Trauma Surgery, VU University Medical Center, P.O. Box 7057, 1007MB Amsterdam, The Netherlands.
J Tissue Viability. 2009 Aug;18(3):80-7. doi: 10.1016/j.jtv.2009.02.005. Epub 2009 Apr 9.
The maggots of Lucilia sericata are successfully used as a treatment for infected wounds. Many articles are published about possible direct antibacterial properties of maggots and their excretions/secretions (ES), but with different results. The present study reinvestigates the susceptibility of six bacterial strains to maggots and their ES.
Live maggots were added to a bacterial suspension. After incubation for 16 h, the bacterial growth in this suspension was compared with the growth in a suspension without maggots. We tested Instar-1 and Instar-3 maggots and compared nutrient broths. A turbidimetric assay investigated the antibacterial activity of ES. Finally, we compared the bacterial growth of Gram-positive and Gram-negative bacteria.
The test with live maggots showed an increase of bacterial growth. Instar-1 maggots stimulated more bacterial growth than Instar-3 maggots, as well as the use of a more nutritious broth. The turbidimetric assay showed no inhibition of bacterial growth. For all bacteria, except Pseudomonas aeruginosa, an increase in bacterial growth was shown.
There is no direct antibacterial effect of maggots and/or ES in vitro, however in clinical observations maggot therapy is successful. More research is needed to focus on possible indirect antibacterial activity, such as an immune-related effect.
丝光绿蝇的蛆已成功用于治疗感染伤口。关于蛆及其排泄物/分泌物（ES）可能的直接抗菌特性，已有许多文章发表，但结果各异。本研究重新调查了六种细菌菌株对蛆及其ES的敏感性。
将活蛆添加到细菌悬液中。孵育16小时后，将该悬液中的细菌生长情况与无蛆悬液中的生长情况进行比较。我们测试了1龄和3龄蛆，并比较了营养肉汤。采用比浊法研究ES的抗菌活性。最后，我们比较了革兰氏阳性菌和革兰氏阴性菌的细菌生长情况。
活蛆试验显示细菌生长增加。1龄蛆比3龄蛆更能刺激细菌生长，使用营养更丰富的肉汤时也是如此。比浊法显示没有抑制细菌生长。除铜绿假单胞菌外，所有细菌的细菌生长均有增加。
蛆和/或ES在体外没有直接抗菌作用，然而在临床观察中蛆疗法是成功的。需要更多研究关注可能的间接抗菌活性，如免疫相关效应。
