丝氨酸棕榈酰转移酶亚基1存在于内质网、细胞核和粘着斑中，并在细胞形态中发挥作用。
Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphology.
作者信息
Wei Jia, Yerokun Tokunbo, Leipelt Martina, Haynes Chris A, Radhakrishna Harish, Momin Amin, Kelly Samuel, Park Hyejung, Wang Elaine, Carton Jill M, Uhlinger David J, Merrill Alfred H
机构信息
Petit Institute for Bioengineering and Bioscience, Atlanta, GA 30332, USA.
出版信息
Biochim Biophys Acta. 2009 Aug;1791(8):746-56. doi: 10.1016/j.bbalip.2009.03.016. Epub 2009 Apr 9.
Serine palmitoyltransferase (SPT) has been localized to the endoplasmic reticulum (ER) by subcellular fractionation and enzymatic assays, and fluorescence microscopy of epitope-tagged SPT; however, our studies have suggested that SPT subunit 1 might be present also in focal adhesions and the nucleus. These additional locations have been confirmed by confocal microscopy using HEK293 and HeLa cells, and for focal adhesions by the demonstration that SPT1 co-immunoprecipitates with vinculin, a focal adhesion marker protein. The focal adhesion localization of SPT1 is associated with cell morphology, and possibly cell migration, because it is seen in most cells before they reach confluence but disappears when they become confluent, and is restored by a standard scratch-wound healing assay. Conversely, elimination of SPT1 using SPTLC1 siRNA causes cell rounding. Thus, in addition to its "traditional" localization in the ER for de novo sphingolipid biosynthesis, SPT1 is present in other cellular compartments, including focal adhesions where it is associated with cell morphology.
通过亚细胞分级分离和酶活性测定，以及对表位标记的丝氨酸棕榈酰转移酶（SPT）进行荧光显微镜观察，已将SPT定位于内质网（ER）；然而，我们的研究表明，SPT亚基1也可能存在于粘着斑和细胞核中。使用HEK293和HeLa细胞通过共聚焦显微镜证实了这些额外的定位，对于粘着斑，通过证明SPT1与粘着斑标记蛋白纽蛋白共免疫沉淀来证实。SPT1在粘着斑的定位与细胞形态相关，可能还与细胞迁移相关，因为在大多数细胞汇合前都能看到它，但当细胞汇合时它就消失了，并且通过标准的划痕伤口愈合试验可以恢复。相反，使用SPTLC1 siRNA消除SPT1会导致细胞变圆。因此，除了其在内质网中进行从头鞘脂生物合成的“传统”定位外，SPT1还存在于其他细胞区室中，包括与细胞形态相关的粘着斑。
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