van 't Hof R, Demel R A, Keegstra K, de Kruijff B
Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands.
FEBS Lett. 1991 Oct 21;291(2):350-4. doi: 10.1016/0014-5793(91)81318-3.
The interactions of fragments of the transit peptide of ribulose-1,5-bisphosphate carboxylase/oxygenase with lipid monolayers was studied in order to investigate the possible involvement of the membrane lipids in the protein import process. The fragments are surface active and have a differential ability to insert in lipid monolayers. The fragments have a preference for the chloroplast galacto- and sulpholipids and phosphatidylglycerol and interact with envelope membrane lipid extracts. These results suggest that probably transit peptide-lipid interactions are involved in the chloroplast protein import process.
为了研究膜脂在蛋白质导入过程中可能的作用，对1,5-二磷酸核酮糖羧化酶/加氧酶转运肽片段与脂质单分子层的相互作用进行了研究。这些片段具有表面活性，并且在脂质单分子层中的插入能力存在差异。这些片段优先与叶绿体半乳糖脂、硫脂和磷脂酰甘油结合，并与包膜膜脂提取物相互作用。这些结果表明，转运肽-脂质相互作用可能参与了叶绿体蛋白质的导入过程。
