Du Ying, Xu Yanjun, Ding Ling, Yao Haomi, Yu Hong, Zhou Tianhua, Si Jianmin
Gastroenterology Laboratory, Clinical Research Institute, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016 Hangzhou, People's Republic of China.
J Gastroenterol. 2009;44(6):556-61. doi: 10.1007/s00535-009-0037-7. Epub 2009 Apr 11.
Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation.
The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay.
MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells.
These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.
人微小RNA - 141（miR - 141）是miR - 200家族的成员，据报道与多种人类恶性肿瘤有关。然而，miR - 141是否参与胃癌的发病机制仍不清楚。因此，我们检测了miR - 141在胃癌组织中的表达以及miR - 141过表达对癌细胞增殖的影响。
通过定量实时PCR检测35对配对的胃癌组织和相邻非肿瘤组织以及5种胃癌细胞系中miR - 141的表达水平。采用MTT（3 - [4,5 - 二甲基噻唑 - 2 - 基] - 2,5 - 二苯基溴化四氮唑）法检测转染了miRNA前体的MGC - 803细胞的生长情况。
与配对的相邻非肿瘤组织相比，80%（28/35）的原发性胃癌组织中miR - 141显著下调（P < 0.01）。在几种人胃癌细胞系如MGC - 803、HGC - 27、SGC - 7901和BGC - 823细胞中也发现miR - 141的表达大幅降低。用其前体过表达miR - 141可显著抑制胃癌细胞的增殖。
这些结果表明，miR - 141可能通过其对细胞增殖的抑制作用参与胃癌的发生发展。
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