[人脐带间充质干细胞治疗骨不连的临床研究]

[Clinical study on treatment of bone nonunion with MSCs derived from human umbilical cord].

作者信息

Qu Zhiguo, Mi Shufeng, Fang Guojun

机构信息

Department of Orthopedic Surgery, the Central Hospital of Siping, Siping Jilin, 136000, PR China.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Mar;23(3):345-7.

PMID:19366151

Abstract

OBJECTIVE

To investigate the clinical effect of MSCs transplantation derived from human umbilical cord on bone nonunion.

METHODS

From December 2005 to December 2007, 72 patients with traumatic bone nonunion were treated. Auto-iliac bone transplantation was used in 36 patients (group A), including 27 males and 9 females, aging (34.0 +/- 2.1) years; including 18 cases of femoral fracture and 18 cases of tibia fracture; and the time of bone nonunion being (9.1 +/- 1.7) months. Percutaneous MSCs transplantation derived from human umbilical cord was used in 36 patients (group B), including 28 males and 8 females, aging (36.0 +/- 1.6) years; including 18 cases of femoral fracture and 18 cases of tibia fracture; and the time of bone nonunion being (6.4 +/- 1.9) months. There were no statistically significant differences in general data between two groups (P > 0.05). In group A, the site of bone nonunion was filled with relevant auto-iliac bone. In group B, the mixture of 6-8 mL platelet-rich plasma prepared by centrifugalizing venous blood and 1 x (10(6)-10(7)) P5 MSCs extracted from human umbilical cord denoted by volunteers was injected into the region of bone nonunion with 0.2 g demineralized bone powder.

RESULTS

Incision healed by first intention in group A. No puncture, deep infection, rejection and general fever reaction occurred in group B. All patients in two groups were followed up for (13.2 +/- 4.6) months. No loosening and breakage of internal fixation were observed in two groups. The motility and function of hip, knee and ankle were good. The time of bone union was (10.3 +/- 2.8) months in group A and (5.6 +/- 0.8) months in groups B, showing significant difference between two groups (P < 0.05).

CONCLUSION

The percutaneous MSCs transplantation derived from human umbilical cord is more effective on bone nonunion than the traditional treatment, it is easily-to-operate, safe, reliable, and rapid for union. It is one of effective methods in treating bone nonunion.

摘要

目的

探讨人脐带间充质干细胞移植治疗骨不连的临床效果。

方法

选取2005年12月至2007年12月收治的72例创伤性骨不连患者。36例患者采用自体髂骨移植（A组），其中男27例，女9例，年龄（34.0±2.1）岁；股骨骨折18例，胫骨骨折18例；骨不连时间为（9.1±1.7）个月。36例患者采用经皮人脐带间充质干细胞移植（B组），其中男28例，女8例，年龄（36.0±1.6）岁；股骨骨折18例，胫骨骨折18例；骨不连时间为（6.4±1.9）个月。两组一般资料比较差异无统计学意义（P＞0.05）。A组将自体髂骨填充于骨不连部位。B组将静脉血经离心制备的6～8 mL富血小板血浆与志愿者捐献的人脐带中提取的1×（10⁶～10⁷）个P5间充质干细胞混合，并加入0.2 g脱矿骨粉，注入骨不连部位。