Nascimbeni Michelina, Perié Leïla, Chorro Laurent, Diocou Seckou, Kreitmann Louis, Louis Stéphanie, Garderet Laurent, Fabiani Bettina, Berger Anne, Schmitz Jürgen, Marie Jean-Pierre, Molina Thierry Jo, Pacanowski Jérôme, Viard Jean-Paul, Oksenhendler Eric, Beq Stéphanie, Abehsira-Amar Orly, Cheynier Rémi, Hosmalin Anne
Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8104, Paris, France.
Blood. 2009 Jun 11;113(24):6112-9. doi: 10.1182/blood-2008-07-170803. Epub 2009 Apr 14.
We characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with high proviral loads. Moreover, although undetectable in most HIV- patients, interferon-alpha (IFN-alpha) production was evidenced in situ and by flow cytometry in most HIV+ patients. IFN-alpha was located in the marginal zone. Surprisingly, IFN-alpha colocalized only with few pDCs, but rather with other cells, including T and B lymphocytes, mDCs, and macrophages. Therefore, pDCs accumulated in spleens from HIV+ patients with high proviral loads, but they did not seem to be the main IFN-alpha producers.
我们对人脾脏中浆细胞样树突状细胞（pDCs）的定位、表型及一些功能进行了表征。pDCs定位于边缘区和动脉周围区域。在红髓中也发现了一些pDCs。通过表型标记，pDCs呈未成熟状态，这与其在功能试验中内化葡聚糖的能力一致。在患有血小板减少性紫癜的HIV感染患者的脾脏中，这些特征未受影响。然而，在一些HIV阳性患者中观察到pDCs的积聚，但髓样树突状细胞（mDCs）未出现积聚，这与高病毒载量相关。此外，虽然在大多数HIV阴性患者中检测不到，但在大多数HIV阳性患者中通过原位检测和流式细胞术证实了α干扰素（IFN-α）的产生。IFN-α位于边缘区。令人惊讶的是，IFN-α仅与少数pDCs共定位，而是与其他细胞共定位，包括T和B淋巴细胞、mDCs及巨噬细胞。因此，在病毒载量高的HIV阳性患者的脾脏中pDCs积聚，但它们似乎不是主要的IFN-α产生细胞。
