Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
Phytother Res. 2009 Dec;23(12):1708-12. doi: 10.1002/ptr.2817.
According to our previous study, Ginkgo biloba extract (GBE) suppresses IL-1beta-induced MUC5AC gene expression in NCI-H292 cells via the ERK and p38 MAPK pathways. This study sought to identify which ingredients of GBE suppress IL-1beta-induced MUC5AC gene expression in NCI-H292 cells and to examine which MAPKs are related to MUC5AC gene suppression for each ingredient. After the cells were pretreated with each ingredient and treated with IL-1beta (10 ng/mL), MUC5AC mRNA expression was determined by RT-PCR and real-time PCR. The results showed that kaempferol (KP) and quercetin (QC) suppressed MUC5AC mRNA expression in a dose-dependent manner, both with significant inhibition starting from 40 microm (equal concentration to about a twelfth or thirteenth dose of GBE). MAPK proteins were determined by western blot analysis after pretreatment with KP, QC and GBE. All three suppressed the phosphorylation of ERK and p38 kinases. In conclusion, the data suggested that KP and QC, essential ingredients in GBE, may overcome the dose problem of GBE and play a valuable role, clinically, in controlling mucin hypersecretion in airway inflammation.
根据我们之前的研究，银杏叶提取物（GBE）通过 ERK 和 p38 MAPK 通路抑制 NCI-H292 细胞中 IL-1β诱导的 MUC5AC 基因表达。本研究旨在确定 GBE 的哪些成分抑制 NCI-H292 细胞中 IL-1β诱导的 MUC5AC 基因表达，并研究对于每种成分，哪些 MAPKs 与 MUC5AC 基因抑制有关。用各成分预处理细胞后，用 IL-1β（10ng/ml）处理细胞，通过 RT-PCR 和实时 PCR 测定 MUC5AC mRNA 表达。结果表明，山奈酚（KP）和槲皮素（QC）呈剂量依赖性抑制 MUC5AC mRNA 表达，从 40μm（相当于 GBE 约十二分之一或十三分之一剂量）开始有显著抑制作用。用 KP、QC 和 GBE 预处理后，通过 Western blot 分析确定 MAPK 蛋白。这三种方法均抑制 ERK 和 p38 激酶的磷酸化。总之，数据表明，GBE 的重要成分 KP 和 QC 可能克服 GBE 的剂量问题，并在控制气道炎症中粘蛋白过度分泌方面具有重要的临床作用。
