吸血蝙蝠圆叶叶口蝠唾液腺中的纤溶酶原激活物家族：克隆与表达

The plasminogen activator family from the salivary gland of the vampire bat Desmodus rotundus: cloning and expression.

作者信息

Krätzschmar J, Haendler B, Langer G, Boidol W, Bringmann P, Alagon A, Donner P, Schleuning W D

机构信息

Research Laboratories of Schering AG Berlin, F.R.G.

出版信息

Gene. 1991 Sep 15;105(2):229-37. doi: 10.1016/0378-1119(91)90155-5.

DOI:10.1016/0378-1119(91)90155-5

PMID:1937019

Abstract

Complementary DNAs coding for four Desmodus rotundus salivary plasminogen activators (DSPAs) were isolated and characterized. The predicted amino acid sequences display structural features also found in tissue-type plasminogen activator. The largest forms (DSPA alpha 1 and -alpha 2) contain a signal peptide, a finger (F), an epidermal growth factor (EGF), a kringle, and a serine protease domain, whereas DSPA beta and -gamma lack the F and F-EGF domains, respectively. Additional differences between the four forms suggest that distinct genes code for the members of the DSPA family. Transfection of DSPA-encoding cDNAs, placed under the control of the simian virus 40 late promoter, into COS-1 cells resulted in the secretion of highly fibrin-dependent PAs.

摘要

编码四种圆叶吸血蝠唾液纤溶酶原激活剂（DSPAs）的互补DNA被分离并进行了表征。预测的氨基酸序列显示出在组织型纤溶酶原激活剂中也存在的结构特征。最大的形式（DSPAα1和-α2）包含一个信号肽、一个指状结构域（F）、一个表皮生长因子（EGF）、一个kringle结构域和一个丝氨酸蛋白酶结构域，而DSPAβ和-γ分别缺少F和F-EGF结构域。这四种形式之间的其他差异表明，不同的基因编码DSPA家族的成员。将置于猿猴病毒40晚期启动子控制下的编码DSPA的cDNA转染到COS-1细胞中，导致分泌高度依赖纤维蛋白的纤溶酶原激活剂。