Petri T, Langer G, Bringmann P, Cashion L, Shallow S, Schleuning W D, Donner P
Research Laboratories of Schering AG, Berlin, Germany.
J Biotechnol. 1995 Feb 21;39(1):75-83. doi: 10.1016/0168-1656(94)00146-4.
Salivary plasminogen activator from the vampire bat Desmodus rotundus (DSPA alpha 1) is a promising new thrombolytic agent. Continuous growth of a stably transfected, methotrexate amplified, dhfr- CHO cell line yields up to 60 mg l-1 of DSPA alpha 1. Utilizing an engineered baculovirus 10 mg l-1 were produced in batches of Sf9 insect cells. Recombinant DSPA alpha 1 is purified from both sources using a one-step purification protocol. Although differences in glycosylation were detected, enzymatic activity and fibrin cofactor dependency are unaffected when DSPA alpha 1 derived from the two expression systems is compared.
吸血蝙蝠（Desmodus rotundus）的唾液纤溶酶原激活剂（DSPAα1）是一种很有前景的新型溶栓剂。稳定转染、经甲氨蝶呤扩增的dhfr-CHO细胞系持续培养可产生高达60毫克/升的DSPAα1。利用一种工程杆状病毒，在Sf9昆虫细胞批次培养中可产生10毫克/升。重组DSPAα1可通过一步纯化方案从这两种来源中进行纯化。尽管检测到糖基化存在差异，但比较源自两种表达系统的DSPAα1时，其酶活性和对纤维蛋白辅因子的依赖性不受影响。
