Chen Fu-Lun, Luh Hui-Tzung, Hsiao Yu-Cheng
Department of Internal Medicine, Division of Infectious Diseases, Taipei Municipal Wan Fang Hospital, Taipei Medical University, Taipei 11031, Taiwan.
Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
Polymers (Basel). 2021 Aug 4;13(16):2587. doi: 10.3390/polym13162587.
We reveal a novel design for dye-doped liquid crystal (DDLC) microfluidic biosensing chips in the polydimethylsiloxane material. With this design chip, the orientation of DDLCs was affected by the interface between the walls of the channels and DDLCs. When the inside of a channel was coated with an N,N-dimethyl-n-octadecyl-3-aminopropyltrimethoxysilyl chloride (DMOAP) alignment layer, the DDLCs oriented homeotropically in a homeotropic (H) state under cross-polarized microscopy. After immobilization of antigens with antibodies on the alignment layer-coated microchannel walls, the optical intensity of the DDLC change from the dark H state to the bright planar (P) state. Using pressure-driven flow, the binding of antigens/antibodies to the DDLCs could be detected in an experimental sequential order. The microfluidic DDLCs were tested by detecting bovine serum albumin (BSA) and its immune-responses of antigens/antibodies. We proved that this immunoassay chip was able to detect BSA antigens/antibodies pairs with the detection limit about 0.5 µg/mL. The novel DDLC chip was shown to be a simple, multi-detection device, and label-free microfluidic chips are presented.
我们展示了一种用于聚二甲基硅氧烷材料中染料掺杂液晶（DDLC）微流控生物传感芯片的新颖设计。采用这种设计的芯片，DDLC的取向受通道壁与DDLC之间界面的影响。当通道内部涂有N，N - 二甲基 - n - 十八烷基 - 3 - 氨丙基三甲氧基硅烷氯化物（DMOAP）取向层时，在交叉偏振显微镜下，DDLC以垂直排列（H）状态垂直取向。在用抗体将抗原固定在涂有取向层的微通道壁上后，DDLC的光强度从暗的H状态变为亮的平面（P）状态。利用压力驱动流，可以按实验顺序检测抗原/抗体与DDLC的结合。通过检测牛血清白蛋白（BSA）及其抗原/抗体的免疫反应来测试微流控DDLC。我们证明这种免疫分析芯片能够检测BSA抗原/抗体对，检测限约为0.5μg/mL。新型DDLC芯片被证明是一种简单的多检测装置，并展示了无标记微流控芯片。
