Liu Tao, Long Lili, Tang Tian, Xia Qingjie, Liu Jin, He Gang, Qiao Xiaoming
Department of Otorhinolaryngology, West China Hospital of Sichuan University, No. 37 Guo-Xue-Xiang, 610041, Chengdu, Sichuan, People's Republic of China.
Cell Mol Neurobiol. 2009 Jul;29(5):739-45. doi: 10.1007/s10571-009-9399-x. Epub 2009 Apr 16.
Endothelin-1 (ET-1) is a peptide with various biological functions, such as vasoconstriction and cell proliferation. ET-1 was reported to be widely distributed throughout the animal body, including nervous system. The expression and localization of ET-1 and its receptors [endothelin type-A receptor (ETAR) and endothelin type-B receptor (ETBR)] in the spiral ganglion neurons have not been reported before. In this study, their presence in the mouse spiral ganglion neurons was detected at mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) technique and immunohistochemistry, respectively. RT-PCR analysis indicated that ET-1, ETAR, and ETBR genes were expressed in the mouse spiral ganglion tissues. Immunohistochemical experiments demonstrated that ET-1 and ETAR were predominantly immunoreactive in the cytoplasm, while ETBR was mainly immunostained in the nucleus of the neuron bodies. The present results suggest that ET-1 may play physiological roles in the spiral ganglion cells via ETAR and ETBR.
内皮素 -1（ET-1）是一种具有多种生物学功能的肽，如血管收缩和细胞增殖。据报道，ET-1广泛分布于动物全身，包括神经系统。此前尚未有关于ET-1及其受体[内皮素A型受体（ETAR）和内皮素B型受体（ETBR）]在螺旋神经节神经元中的表达和定位的报道。在本研究中，分别通过逆转录 - 聚合酶链反应（RT-PCR）技术和免疫组织化学在mRNA和蛋白质水平检测了它们在小鼠螺旋神经节神经元中的存在情况。RT-PCR分析表明，ET-1、ETAR和ETBR基因在小鼠螺旋神经节组织中表达。免疫组织化学实验表明，ET-1和ETAR主要在细胞质中具有免疫反应性，而ETBR主要在神经元胞体的细胞核中被免疫染色。目前的结果表明，ET-1可能通过ETAR和ETBR在螺旋神经节细胞中发挥生理作用。
