Lapenkov M I, Bogatyreva E A, Aleksandrova V Iu, Smirnova V K, Nikolaeva T L
Sud Med Ekspert. 2009 Jan-Feb;52(1):40-3.
A one-step modification ofimmunoenzyme assay (dot variant) is proposed for the detection of ABH-Lewis antigens in human discharges (saliva, sperm, vaginal discharge). Sensitivity and specificity of the new method is higher than those of other methods currently used in forensic medicine for the detection of antigens in human body discharges; its other advantages over routine techniques include procedural simplicity, high performance, and the possibility of conducting series measurements with minimal labour inputs. Moreover, it provides information not only about group characteristic but also about category of discharge. The analysis is carried out in supernatant fractions which permits to reserve cellular material for molecular-genetic studies.
本文提出了一种免疫酶测定法（斑点变体）的一步改良法，用于检测人体分泌物（唾液、精液、阴道分泌物）中的ABH-刘易斯抗原。新方法的灵敏度和特异性高于目前法医学中用于检测人体分泌物中抗原的其他方法；与常规技术相比，它的其他优点包括操作简单、性能高，以及能够以最少的人力投入进行系列测量。此外，它不仅能提供血型特征信息，还能提供分泌物类型信息。分析是在上清液部分进行的，这样可以保留细胞材料用于分子遗传学研究。
