Hultman P, Johansson U
Department of Pathology, Linköping University, University Hospital, Sweden.
Food Chem Toxicol. 1991 Sep;29(9):633-8. doi: 10.1016/0278-6915(91)90146-x.
The effect of mercuric chloride on mitogen-induced DNA synthesis was investigated using lymphocytes from SJL and DBA mice, which are known to be susceptible and resistant to induction of autoimmunity by mercury, respectively. Treatment of SJL mice with 5 ppm mercuric chloride in their drinking-water for 2 wk resulted in a two- to three-fold increase of concanavalin A and lipopolysaccharide-induced thymidine incorporation in splenocytes. Mitogen-induced thymidine incorporation in splenocytes from identically treated DBA mice was not significantly different from that seen in controls. In vitro treatment of splenocytes from SJL mice with mercury caused a dose-dependent increase of concanavalin A-, lipopolysaccharide and phytohaemagglutinin-induced thymidine incorporation. This effect was optimal when 10(-7)-10(-8) M-mercuric chloride were added as a pulse 1 hr before the addition of mitogens, whereas higher and lower concentrations were less effective. The mitogen-induced DNA synthesis in splenocytes from DBA mice was either not affected by the addition of mercuric chloride or decreased by higher mercury concentrations. The addition of mercury to thymocytes from DBA and SJL mice caused a slight and moderate increase, respectively, in concanavalin A-induced thymidine incorporation.
使用分别对汞诱导自身免疫敏感和抗性的SJL和DBA小鼠的淋巴细胞，研究了氯化汞对有丝分裂原诱导的DNA合成的影响。用5 ppm氯化汞处理SJL小鼠的饮用水2周，导致脾细胞中伴刀豆球蛋白A和脂多糖诱导的胸苷掺入增加两到三倍。相同处理的DBA小鼠脾细胞中有丝分裂原诱导的胸苷掺入与对照组无显著差异。用汞对SJL小鼠的脾细胞进行体外处理，导致伴刀豆球蛋白A、脂多糖和植物血凝素诱导的胸苷掺入呈剂量依赖性增加。当在添加有丝分裂原前1小时脉冲添加10(-7)-10(-8) M氯化汞时，这种效果最佳，而更高和更低的浓度效果较差。添加氯化汞对DBA小鼠脾细胞中有丝分裂原诱导的DNA合成要么没有影响，要么在汞浓度较高时降低。向DBA和SJL小鼠的胸腺细胞中添加汞，分别使伴刀豆球蛋白A诱导的胸苷掺入略有和适度增加。
