口腔鳞状细胞癌浸润前沿间质促纤维增生及肌成纤维细胞表现的意义
Significance of stromal desmoplasia and myofibroblast appearance at the invasive front in squamous cell carcinoma of the oral cavity.
作者信息
Kawashiri Shuichi, Tanaka Akira, Noguchi Natsuyo, Hase Takashi, Nakaya Hiromitsu, Ohara Teruhisa, Kato Koroku, Yamamoto Etsuhide
机构信息
Department of Oral and Maxillofacial Surgery, Division of Cancer Science, Graduate School of Medical Science, Kanazawa University, Kanazawa Ishikawa 920-8640, Japan.
出版信息
Head Neck. 2009 Oct;31(10):1346-53. doi: 10.1002/hed.21097.
BACKGROUND
Tumor invasion involves complex interactions between tumor and stromal cells. We examined the extent of connective tissue in the tumor stroma and whether myofibroblasts play a role in assisting cancer invasion and metastasis.
METHODS
Biopsy materials from 84 patients with oral squamous cell carcinoma (SCC) were used. We compared data from intrastromal collagen fibers using Azan staining, immunohistochemical identification of myofibroblasts by cytoskeletal markers, alpha-smooth muscle actin, vimentin, desmin, and clinicopathological parameters. Clinical outcome was compared by 5-year survival rate.
RESULTS
There were high levels of stromal collagen fibers in invasive tumors. Myofibroblast appearance increased with increasing tumor invasiveness. Lymph node metastasis occurred more frequently in the myofibroblast-positive group, and the survival rate was significantly poorer in this group.
CONCLUSIONS
Fibrous stroma in SCC appeared to have a desmoplastic response. However, an independent invasive mechanism may regulate the stroma, with tumor desmoplasia occurring in highly developed, invasive tumors.
背景
肿瘤侵袭涉及肿瘤细胞与基质细胞之间复杂的相互作用。我们研究了肿瘤基质中结缔组织的程度以及肌成纤维细胞在协助癌症侵袭和转移中是否发挥作用。
方法
使用来自84例口腔鳞状细胞癌（SCC）患者的活检材料。我们使用偶氮染色比较了基质内胶原纤维的数据，通过细胞骨架标记物、α-平滑肌肌动蛋白、波形蛋白、结蛋白对肌成纤维细胞进行免疫组织化学鉴定，并比较临床病理参数。通过5年生存率比较临床结局。
结果
侵袭性肿瘤中基质胶原纤维水平较高。肌成纤维细胞的出现随着肿瘤侵袭性的增加而增加。肌成纤维细胞阳性组淋巴结转移更频繁，且该组生存率明显较差。
结论
SCC中的纤维性基质似乎有促结缔组织增生反应。然而，一种独立的侵袭机制可能调节基质，肿瘤促结缔组织增生发生在高度发展的侵袭性肿瘤中。
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