Gender differences in delay-discounting under mild food restriction.

Impulsivity, a core symptom of attention-deficit hyperactivity disorder (ADHD), is tested in animal models by delay-discounting tasks. So far, mainly male subjects have been used in this paradigm at severe levels of food restriction. Here we studied the impulsive behaviour of CD-1 adult male and female mice at mild levels of food restriction. Mice maintained at 90 +/- 5% of ad libitum bodyweight, were tested in operant chambers provided with nose-poking holes. Nose poking in one hole resulted in the immediate delivery of one food pellet (small-soon, SS), whereas nose poking in the other hole delivered five food pellets after a delay (large-late, LL), which was increased progressively each day (0-150 s). Two subgroups emerged: individuals that shifted at short delays ("steep") and individuals that did not shift, even at the highest delays ("flat"). Analysis showed that "steep" females shifted at shorter delays than "steep" males, while no difference existed between males and females within the "flat" sub-population. In home-cage circadian activity as well as in a novelty-seeking test, females were more active than males. It can be concluded from these results that female mice are more impulsive than male mice under mild food restriction. This is in contrast with findings in earlier studies with more severe food restriction. Therefore, an alternative explanation is that females are more explorative, and that different features might be tested in delay-discounting paradigms, depending on restriction levels.