Mnasria K, Lagaraine C, Manaa J, Lebranchu Y, Oueslati R
UR immuno microbiologie environnementale et cancérogenèse, faculté des sciences de Bizerte, Tunisie.
Pathol Biol (Paris). 2011 Jun;59(3):e29-35. doi: 10.1016/j.patbio.2009.03.002. Epub 2009 Apr 16.
The results provide new insights into the role of IL-2/IL-2R pathway in DC. We report that stimulation of human monocyte-derived DC with LPS strongly upregulated CD25 (α chain of the IL-2R) expression. In addition, by using a humanized monoclonal antibody against CD25, we demonstrated that the IL-2 signalling in DC upregulated both IL-12 and γIFN production but decreased IL-10 synthesis. Anti-CD25 treatment reduced the ability of LPS-DC to induce allogeneic CD4(+) T cell proliferation as compared to LPS-matured DC. In addition, LPS-matured DC treated with IL-2 had a higher allostimulatory capacity compared to LPS-DC. We also found that LPS-matured DC produced IL-2. Thus, IL-2 seems to contribute actively to DC activation through an autocrine pathway. Moreover, IL-2 pathway in DC is involved in T helper priming. These findings might be useful for protocols in cellular therapy and a valuable tool to understand graft rejection versus the acquisition of peripheral tolerance.
这些结果为白细胞介素-2/白细胞介素-2受体（IL-2/IL-2R）通路在树突状细胞（DC）中的作用提供了新的见解。我们报告称，用脂多糖（LPS）刺激人单核细胞衍生的DC可强烈上调CD25（IL-2R的α链）的表达。此外，通过使用抗CD25的人源化单克隆抗体，我们证明DC中的IL-2信号传导上调了IL-12和γ干扰素的产生，但降低了IL-10的合成。与LPS成熟的DC相比，抗CD25处理降低了LPS-DC诱导同种异体CD4(+) T细胞增殖的能力。此外，与LPS-DC相比，用IL-2处理的LPS成熟的DC具有更高的同种异体刺激能力。我们还发现LPS成熟的DC产生IL-2。因此，IL-2似乎通过自分泌途径积极促进DC的激活。此外，DC中的IL-2通路参与辅助性T细胞的启动。这些发现可能对细胞治疗方案有用，并且是理解移植排斥与外周耐受获得的有价值工具。
