Rao K V, Bhide S V, Khadke D T, Pendse M D
Carcinogenesis Division Cancer Research Institute, Tata Memorial Centre, Bombay, India.
Indian J Cancer. 1991 Jun;28(2):84-91.
Newborn Syrian hamster dermal fibroblasts in primary culture have high plating efficiency, manifest as homogeneous contact-sensitive monolayer in mass cultures and above all possesses a short life-span of 15-20 days in culture. These cells are quite sensitive to mutagenic carcinogens. The DMBA treatment induced multi-layer and foci formation and growth in soft-agar suggesting that immortal variants can be obtained by treatment with carcinogens.
原代培养的新生叙利亚仓鼠皮肤成纤维细胞具有较高的接种效率，在大规模培养中表现为均匀的接触敏感单层，最重要的是在培养中寿命较短，仅15 - 20天。这些细胞对诱变致癌物相当敏感。经二甲基苯并蒽（DMBA）处理后，细胞形成多层和集落，并在软琼脂中生长，这表明通过致癌物处理可以获得永生变体。
