Rao K V, D'Souza A V, Bhide S V
Neoplasma. 1983;30(1):35-42.
Fine mince of embryonic rat tongue suspended in medium and transferred to culture vessel adhere immediately to surface. Epithelial and fibroblastic cell outgrowth seen in the first few days was overgrown with fibroblasts later. Evidence is presented that these fibroblasts are sensitive to post-confluence inhibition of division and exhibit several properties of normal fibroblasts including limited lifespan. This system may serve as a simple in vitro model for oral carcinogenesis studies.
将胚胎大鼠舌头的精细切碎物悬浮在培养基中并转移到培养容器中，它会立即附着在表面。最初几天可见上皮细胞和成纤维细胞生长，之后成纤维细胞过度生长。有证据表明，这些成纤维细胞对汇合后分裂抑制敏感，并表现出正常成纤维细胞的几种特性，包括有限的寿命。该系统可作为口腔癌发生研究的简单体外模型。
