Wengenack Nancy L, Binnicker Matthew J
Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 55905, USA.
Clin Chest Med. 2009 Jun;30(2):391-408, viii. doi: 10.1016/j.ccm.2009.02.014.
This article summarizes the current state of the art in molecular diagnostic methods for the detection and identification of fungi as performed in the mycology laboratory. The advantages and limitations of current molecular testing methods are evaluated, with examples provided from the recent literature. Finally, commentary is provided on the future of molecular fungal diagnostics, including the potential role of multiplex detection assays, microarrays, and the molecular determination of antifungal resistance directly from clinical specimens.
本文总结了真菌学实验室用于检测和鉴定真菌的分子诊断方法的当前技术水平。评估了当前分子检测方法的优缺点,并列举了近期文献中的实例。最后,对分子真菌诊断的未来进行了评论,包括多重检测分析、微阵列以及直接从临床标本中进行抗真菌药物耐药性分子测定的潜在作用。