双功能酶UDP-芹糖/UDP-木糖合酶中间体和不稳定产物的实时核磁共振监测
Real-time NMR monitoring of intermediates and labile products of the bifunctional enzyme UDP-apiose/UDP-xylose synthase.
作者信息
Guyett Paul, Glushka John, Gu Xiaogang, Bar-Peled Maor
机构信息
Complex Carbohydrate Research Center, and BioEnergy Science Center, 315 Riverbend Road, University of Georgia, Athens, GA 30602, USA.
出版信息
Carbohydr Res. 2009 Jun 12;344(9):1072-8. doi: 10.1016/j.carres.2009.03.026. Epub 2009 Mar 27.
Abstract
The conversion of UDP-alpha-d-glucuronic acid to UDP-alpha-d-xylose and UDP-alpha-d-apiose by a bifunctional potato enzyme UDP-apiose/UDP-xylose synthase was studied using real-time nuclear magnetic resonance (NMR) spectroscopy. UDP-alpha-d-glucuronic acid is converted via the intermediate uridine 5'-beta-l-threo-pentapyranosyl-4''-ulose diphosphate to UDP-alpha-d-apiose and simultaneously to UDP-alpha-d-xylose. The UDP-alpha-d-apiose that is formed is unstable and is converted to alpha-d-apio-furanosyl-1,2-cyclic phosphate and UMP. High-resolution real-time NMR spectroscopy is a powerful tool for the direct and quantitative characterization of previously undetected transient and labile components formed during a complex enzyme-catalyzed reaction.
摘要
利用实时核磁共振（NMR）光谱研究了双功能马铃薯酶UDP-芹糖/UDP-木糖合酶将UDP-α-D-葡萄糖醛酸转化为UDP-α-D-木糖和UDP-α-D-芹糖的过程。UDP-α-D-葡萄糖醛酸通过中间体尿苷5'-β-L-苏式-五吡喃糖基-4''-酮二磷酸转化为UDP-α-D-芹糖，同时转化为UDP-α-D-木糖。生成的UDP-α-D-芹糖不稳定，会转化为α-D-呋喃芹糖基-1,2-环磷酸酯和UMP。高分辨率实时NMR光谱是直接和定量表征复杂酶催化反应过程中形成的先前未检测到的瞬态和不稳定成分的有力工具。
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