• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Real-time NMR monitoring of intermediates and labile products of the bifunctional enzyme UDP-apiose/UDP-xylose synthase.双功能酶UDP-芹糖/UDP-木糖合酶中间体和不稳定产物的实时核磁共振监测
Carbohydr Res. 2009 Jun 12;344(9):1072-8. doi: 10.1016/j.carres.2009.03.026. Epub 2009 Mar 27.
2
Synthesis, characterization and properties of uridine 5'-( -D-apio-D-furanosyl pyrophosphate).尿苷5'-（-D-阿卓-D-呋喃糖基焦磷酸）的合成、表征及性质
Biochem J. 1973 Jun;133(2):227-41. doi: 10.1042/bj1330227.
3
Transcriptome-guided gene isolation and functional characterization of UDP-xylose synthase and UDP-D-apiose/UDP-D-xylose synthase families from Ornithogalum caudatum Ait.基于转录组的虎眼万年青UDP-木糖合酶和UDP-D-芹糖/UDP-D-木糖合酶家族基因分离及功能鉴定
Plant Cell Rep. 2016 Nov;35(11):2403-2421. doi: 10.1007/s00299-016-2044-5. Epub 2016 Sep 3.
4
Analysis of UDP-D-apiose/UDP-D-xylose synthase-catalyzed conversion of UDP-D-apiose phosphonate to UDP-D-xylose phosphonate: implications for a retroaldol-aldol mechanism.分析 UDP-D-岩藻糖/ UDP-D-木糖合酶催化 UDP-D-岩藻糖磷酸酯向 UDP-D-木糖磷酸酯的转化：对 retroaldol-aldol 机制的启示。
J Am Chem Soc. 2012 Aug 29;134(34):13946-9. doi: 10.1021/ja305322x. Epub 2012 Aug 15.
5
A fluoro analogue of UDP-α-D-glucuronic acid is an inhibitor of UDP-α-D-apiose/UDP-α-D-xylose synthase.尿苷二磷酸-α-D-葡萄糖醛酸的氟代类似物是 UDP-α-D-艾杜糖醛酸/ UDP-α-D-木糖合酶的抑制剂。
Chem Commun (Camb). 2011 Sep 28;47(36):10130-2. doi: 10.1039/c1cc13140k. Epub 2011 Aug 9.
6
The biosynthesis of the branched-chain sugar d-apiose in plants: functional cloning and characterization of a UDP-d-apiose/UDP-d-xylose synthase from Arabidopsis.植物中支链糖D-阿洛糖的生物合成：来自拟南芥的UDP-D-阿洛糖/UDP-D-木糖合酶的功能克隆与特性分析
Plant J. 2003 Sep;35(6):693-703. doi: 10.1046/j.1365-313x.2003.01841.x.
7
Biosynthesis of UDP-xylose and UDP-arabinose in Sinorhizobium meliloti 1021: first characterization of a bacterial UDP-xylose synthase, and UDP-xylose 4-epimerase.苜蓿中华根瘤菌 1021 中 UDP-木糖和 UDP-阿拉伯糖的生物合成：一种细菌 UDP-木糖合酶和 UDP-木糖 4-差向异构酶的首次表征。
Microbiology (Reading). 2011 Jan;157(Pt 1):260-269. doi: 10.1099/mic.0.040758-0. Epub 2010 Sep 16.
8
Functional characterization of the UDP-xylose biosynthesis pathway in Rhodothermus marinus.海栖嗜热放线菌中UDP-木糖生物合成途径的功能表征
Appl Microbiol Biotechnol. 2015 Nov;99(22):9463-72. doi: 10.1007/s00253-015-6683-1. Epub 2015 Jun 2.
9
Identification of a bifunctional UDP-4-keto-pentose/UDP-xylose synthase in the plant pathogenic bacterium Ralstonia solanacearum strain GMI1000, a distinct member of the 4,6-dehydratase and decarboxylase family.鉴定植物病原菌罗尔斯顿氏菌 GMI1000 菌株中一种具有双功能的 UDP-4-酮戊糖/UDP-木糖合酶，该酶是 4,6-脱水酶和脱羧酶家族的一个独特成员。
J Biol Chem. 2010 Mar 19;285(12):9030-40. doi: 10.1074/jbc.M109.066803. Epub 2010 Jan 29.
10
Conversion of UDP-D-glucuronic acid to UDP-D-apiose and UDP-D-xylose by an enzyme isolated from Lemna minor.从小浮萍中分离出的一种酶将UDP-D-葡萄糖醛酸转化为UDP-D-芹糖和UDP-D-木糖。
Biochim Biophys Acta. 1974 Sep 11;364(1):159-72. doi: 10.1016/0005-2744(74)90143-0.

引用本文的文献

1
Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis.解析UDP-芹糖生物合成中糖环收缩的酶促机制。
Nat Catal. 2019 Nov 25;2(12):1115-1123. doi: 10.1038/s41929-019-0382-8.
2
Identification of an apiosyltransferase in the plant pathogen Xanthomonas pisi.鉴定豌豆细菌性叶斑病菌中的一个芹糖基转移酶。
PLoS One. 2018 Oct 18;13(10):e0206187. doi: 10.1371/journal.pone.0206187. eCollection 2018.
3
Correlation of Apiose Levels and Growth Rates in Duckweeds.浮萍中芹糖水平与生长速率的相关性
Front Chem. 2018 Jul 20;6:291. doi: 10.3389/fchem.2018.00291. eCollection 2018.
4
Changes in the abundance of cell wall apiogalacturonan and xylogalacturonan and conservation of rhamnogalacturonan II structure during the diversification of the Lemnoideae.在浮萍科植物多样化过程中，细胞壁阿魏酸半乳聚糖和木半乳聚糖的丰度变化和鼠李半乳糖醛酸 II 结构的保守性。
Planta. 2018 Apr;247(4):953-971. doi: 10.1007/s00425-017-2837-y. Epub 2017 Dec 29.
5
Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi.细菌中UDP-芹糖的合成：海洋光合生物玫瑰双球菌和植物病原菌豌豆黄单胞菌。
PLoS One. 2017 Sep 20;12(9):e0184953. doi: 10.1371/journal.pone.0184953. eCollection 2017.
6
Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage.利用同位素探针研究 UDP-阿比糖/UDP-木糖合酶反应：通过偶联氧化和醛醇裂解的机制证据。
Angew Chem Int Ed Engl. 2017 Feb 20;56(9):2503-2507. doi: 10.1002/anie.201609288. Epub 2017 Jan 19.
7
UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage.UUAT1是一种定位于高尔基体的UDP-糖醛酸转运蛋白，可调节拟南芥种子黏液的多糖组成。
Plant Cell. 2017 Jan;29(1):129-143. doi: 10.1105/tpc.16.00465. Epub 2017 Jan 6.
8
Transcriptome-guided gene isolation and functional characterization of UDP-xylose synthase and UDP-D-apiose/UDP-D-xylose synthase families from Ornithogalum caudatum Ait.基于转录组的虎眼万年青UDP-木糖合酶和UDP-D-芹糖/UDP-D-木糖合酶家族基因分离及功能鉴定
Plant Cell Rep. 2016 Nov;35(11):2403-2421. doi: 10.1007/s00299-016-2044-5. Epub 2016 Sep 3.
9
Functional Characterization of UDP-apiose Synthases from Bryophytes and Green Algae Provides Insight into the Appearance of Apiose-containing Glycans during Plant Evolution.苔藓植物和绿藻中UDP-芹糖合成酶的功能表征有助于深入了解含芹糖聚糖在植物进化过程中的出现情况。
J Biol Chem. 2016 Oct 7;291(41):21434-21447. doi: 10.1074/jbc.M116.749069. Epub 2016 Aug 22.
10
Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433.参与吲哚咔唑AT2433生物合成途径中TDP-氨基脱氧戊糖早期合成的酶的表征
Chembiochem. 2015 Oct 12;16(15):2141-6. doi: 10.1002/cbic.201500365. Epub 2015 Sep 18.

本文引用的文献

1
Identification of labile UDP-ketosugars in Helicobacter pylori, Campylobacter jejuni and Pseudomonas aeruginosa: key metabolites used to make glycan virulence factors.幽门螺杆菌、空肠弯曲菌和铜绿假单胞菌中不稳定UDP-酮糖的鉴定：用于制造聚糖毒力因子的关键代谢物。
Chembiochem. 2006 Dec;7(12):1865-8. doi: 10.1002/cbic.200600298.
2
Developmental Control of Apiogalacturonan Biosynthesis and UDP-Apiose Production in a Duckweed.浮萍中果胶寡糖生物合成和 UDP-阿比糖产生的发育调控
Plant Physiol. 1989 Jul;90(3):972-6. doi: 10.1104/pp.90.3.972.
3
Structure of Plant Cell Walls: VIII. A New Pectic Polysaccharide.植物细胞壁的结构：VIII. 一种新型的果胶多糖。
Plant Physiol. 1978 Sep;62(3):418-22. doi: 10.1104/pp.62.3.418.
4
Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity.幽门螺杆菌FlaA1的结构研究揭示了4,6-脱水酶活性反转的机制。
J Biol Chem. 2006 Aug 25;281(34):24489-95. doi: 10.1074/jbc.M602393200. Epub 2006 May 1.
5
Real-time monitoring of chemical transformations by ultrafast 2D NMR spectroscopy.通过超快二维核磁共振光谱对化学转化进行实时监测。
J Am Chem Soc. 2006 Jan 25;128(3):951-6. doi: 10.1021/ja0564158.
6
Structure and assembly of epiglucan, the extracellular (1-->3;1-->6)-beta-glucan produced by the fungus Epicoccum nigrum strain F19.表葡聚糖的结构与组装，表葡聚糖是由真菌黑附球菌F19菌株产生的胞外（1→3；1→6）-β-葡聚糖。
Carbohydr Res. 2006 Feb 27;341(3):365-73. doi: 10.1016/j.carres.2005.10.013. Epub 2005 Dec 15.
7
Statistical total correlation spectroscopy: an exploratory approach for latent biomarker identification from metabolic 1H NMR data sets.统计全相关光谱法：一种从代谢1H NMR数据集识别潜在生物标志物的探索性方法。
Anal Chem. 2005 Mar 1;77(5):1282-9. doi: 10.1021/ac048630x.
8
The biosynthesis of UDP-galacturonic acid in plants. Functional cloning and characterization of Arabidopsis UDP-D-glucuronic acid 4-epimerase.植物中UDP-半乳糖醛酸的生物合成。拟南芥UDP-D-葡萄糖醛酸4-表异构酶的功能克隆与特性分析。
Plant Physiol. 2004 Dec;136(4):4256-64. doi: 10.1104/pp.104.052365. Epub 2004 Nov 24.
9
Study of flavonoids of Sechium edule (Jacq) Swartz (Cucurbitaceae) different edible organs by liquid chromatography photodiode array mass spectrometry.利用液相色谱-光电二极管阵列-质谱联用技术对佛手瓜（葫芦科）不同可食器官中的黄酮类化合物进行研究。
J Agric Food Chem. 2004 Oct 20;52(21):6510-5. doi: 10.1021/jf040214q.
10
Rhamnogalacturonan II: structure and function of a borate cross-linked cell wall pectic polysaccharide.鼠李半乳糖醛酸聚糖II：一种硼酸交联的细胞壁果胶多糖的结构与功能
Annu Rev Plant Biol. 2004;55:109-39. doi: 10.1146/annurev.arplant.55.031903.141750.

双功能酶UDP-芹糖/UDP-木糖合酶中间体和不稳定产物的实时核磁共振监测

Real-time NMR monitoring of intermediates and labile products of the bifunctional enzyme UDP-apiose/UDP-xylose synthase.

作者信息

Guyett Paul, Glushka John, Gu Xiaogang, Bar-Peled Maor

机构信息

Complex Carbohydrate Research Center, and BioEnergy Science Center, 315 Riverbend Road, University of Georgia, Athens, GA 30602, USA.

出版信息

Carbohydr Res. 2009 Jun 12;344(9):1072-8. doi: 10.1016/j.carres.2009.03.026. Epub 2009 Mar 27.

DOI:10.1016/j.carres.2009.03.026
PMID:19375693
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4000172/
Abstract

The conversion of UDP-alpha-d-glucuronic acid to UDP-alpha-d-xylose and UDP-alpha-d-apiose by a bifunctional potato enzyme UDP-apiose/UDP-xylose synthase was studied using real-time nuclear magnetic resonance (NMR) spectroscopy. UDP-alpha-d-glucuronic acid is converted via the intermediate uridine 5'-beta-l-threo-pentapyranosyl-4''-ulose diphosphate to UDP-alpha-d-apiose and simultaneously to UDP-alpha-d-xylose. The UDP-alpha-d-apiose that is formed is unstable and is converted to alpha-d-apio-furanosyl-1,2-cyclic phosphate and UMP. High-resolution real-time NMR spectroscopy is a powerful tool for the direct and quantitative characterization of previously undetected transient and labile components formed during a complex enzyme-catalyzed reaction.

摘要

利用实时核磁共振（NMR）光谱研究了双功能马铃薯酶UDP-芹糖/UDP-木糖合酶将UDP-α-D-葡萄糖醛酸转化为UDP-α-D-木糖和UDP-α-D-芹糖的过程。UDP-α-D-葡萄糖醛酸通过中间体尿苷5'-β-L-苏式-五吡喃糖基-4''-酮二磷酸转化为UDP-α-D-芹糖，同时转化为UDP-α-D-木糖。生成的UDP-α-D-芹糖不稳定，会转化为α-D-呋喃芹糖基-1,2-环磷酸酯和UMP。高分辨率实时NMR光谱是直接和定量表征复杂酶催化反应过程中形成的先前未检测到的瞬态和不稳定成分的有力工具。