耐核酸酶的甲基膦酸酯-DNA/LNA嵌合寡核苷酸。

Nuclease resistant methylphosphonate-DNA/LNA chimeric oligonucleotides.

作者信息

Nagahama Koji, Veedu Rakesh N, Wengel Jesper

机构信息

Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Odense M, Denmark.

出版信息

Bioorg Med Chem Lett. 2009 May 15;19(10):2707-9. doi: 10.1016/j.bmcl.2009.03.116. Epub 2009 Mar 28.

DOI:10.1016/j.bmcl.2009.03.116

PMID:19375912

Abstract

Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3'-exonucleolytic stability are described. The obtained methylphosphonate-DNA/LNA chimeric oligonucleotides display similarly high RNA affinity and RNA selectivity as a corresponding 9-mer DNA/LNA chimeric oligonucleotide, but much higher resistance towards 3'-exonucleolytic degradation.

摘要

本文描述了含甲基膦酸酯连接和锁核酸（LNA）单体的嵌合9聚体寡核苷酸的合成、它们与互补DNA和RNA的结合亲和力以及它们的3'-核酸外切酶稳定性。所获得的甲基膦酸酯-DNA/LNA嵌合寡核苷酸与相应的9聚体DNA/LNA嵌合寡核苷酸表现出相似的高RNA亲和力和RNA选择性，但对3'-核酸外切酶降解具有更高的抗性。