Ribeiro Letícia Carina, Quincozes-Santos André, Leite Marina Concli, Abib Renata Torres, Kleinkauf-Rocha Juliana, Biasibetti Regina, Rotta Liane Nanci, Wofchuk Susana T, Perry Marcos Luiz Santos, Gonçalves Carlos-Alberto, Gottfried Carmem
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Neurosci Res. 2009 Jul;64(3):330-4. doi: 10.1016/j.neures.2009.04.004. Epub 2009 Apr 17.
Recent studies indicate that caloric restriction (CR) protects the central nervous system from several pathological conditions. The impairment of astroglial cell function, including glutamate uptake, glutamine synthetase (GS) activity and S100B secretion, may contribute to the progression of neurological disorders. The present study aimed to evaluate hippocampal astrocytic changes in response to CR diet, measuring astroglial parameters, such as glutamate uptake, GS activity and the immunocontent of GFAP and S100B. Blood biochemical parameters were also analyzed. Rats (60-day old) were fed ad libitum or on CR diets for 12 weeks. CR-fed rats showed approximately 16% less body weight gain than control rats. The CR diet was able to induce a significant increase in glutamate uptake (23%) and in GS activity (26%). There were no statistically significant differences in the immunocontent of either GFAP or S100B. In summary, the present study indicates that CR also modulates astrocyte functions by increasing glutamate uptake and GS activity, suggesting that CR might exert its neuroprotective effects against brain illness by modulation of astrocytic functions.
近期研究表明，热量限制（CR）可保护中枢神经系统免受多种病理状况的影响。星形胶质细胞功能的损害，包括谷氨酸摄取、谷氨酰胺合成酶（GS）活性和S100B分泌，可能会促进神经系统疾病的进展。本研究旨在评估热量限制饮食对海马星形胶质细胞的影响，测量星形胶质细胞参数，如谷氨酸摄取、GS活性以及胶质纤维酸性蛋白（GFAP）和S100B的免疫含量。还分析了血液生化参数。将60日龄大鼠随意喂食或给予热量限制饮食12周。接受热量限制饮食的大鼠体重增加比对照大鼠少约16%。热量限制饮食能够使谷氨酸摄取量显著增加（23%），GS活性显著增加（26%）。GFAP或S100B的免疫含量没有统计学上的显著差异。总之，本研究表明，热量限制还可通过增加谷氨酸摄取和GS活性来调节星形胶质细胞功能，提示热量限制可能通过调节星形胶质细胞功能对脑部疾病发挥神经保护作用。
