伯纳特柯克斯体中一个独特的I组内含子是一个天然剪接突变体。
A unique group I intron in Coxiella burnetii is a natural splice mutant.
作者信息
Raghavan Rahul, Hicks Linda D, Minnick Michael F
机构信息
Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA.
出版信息
J Bacteriol. 2009 Jun;191(12):4044-6. doi: 10.1128/JB.00359-09. Epub 2009 Apr 17.
Abstract
Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3'-terminal adenine in place of a conserved guanine. Here, we show that, unlike all other group I introns, Cbu.L1917 utilizes a different cofactor for each splicing step and has a decreased self-splicing rate in vitro.
摘要
Cbu.L1917是存在于伯氏考克斯氏体23S rRNA基因中的I组内含子，其3'-末端有一个独特的腺嘌呤取代了保守的鸟嘌呤。在此，我们表明，与所有其他I组内含子不同，Cbu.L1917在每个剪接步骤中使用不同的辅因子，并且在体外自剪接率降低。
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