氨基酸可用性对罗伊氏乳杆菌中维生素B12产生的影响。
Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri.
作者信息
Santos Filipe, Teusink Bas, Molenaar Douwe, van Heck Maurice, Wels Michiel, Sieuwerts Sander, de Vos Willem M, Hugenholtz Jeroen
机构信息
Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO food research, BA Ede, The Netherlands.
出版信息
Appl Environ Microbiol. 2009 Jun;75(12):3930-6. doi: 10.1128/AEM.02487-08. Epub 2009 Apr 17.
Abstract
Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B(12) production and provided new avenues for future improvement of production of vitamin B(12).
摘要
近期的功能基因组学和基因组规模建模方法表明，通过优化培养基可以提高罗伊氏乳杆菌中维生素B12的产量。在此我们表明，一系列系统性的单氨基酸缺失能够显著调节维生素B12的产量，从几乎检测不到的水平（异亮氨酸缺失时）提高到比先前报道水平高20倍的水平（半胱氨酸缺失时）。利用cDNA微阵列实验，我们分析了罗伊氏乳杆菌对缺乏半胱氨酸的培养基的转录反应。结果支持了所观察到的高水平维生素B12产量，并为未来提高维生素B12产量提供了新途径。
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