Department of Biotechnology, Songdo Technopark, Yeonsu-Gu, Incheon, Republic of Korea.
Biochemistry (Mosc). 2011 Apr;76(4):423-6. doi: 10.1134/s0006297911040043.
Glutathione (GSH) is an abundant nonprotein thiol that plays numerous roles within the cell. Previously, we showed that Lactobacillus salivarius has the capacity to mount a glutathione-mediated acid-tolerance response. In the present work we provide evidence of a requirement for GSH by Lactobacillus reuteri and have studied the role of GSH during cell growth. Medium supplementation with 0.5 mM GSH as the sole sulfur source enhanced cell growth, resulting in an increase in glucose consumption, and increased cell GSH and protein contents compared with levels seen in the absence of supplementation. Moreover, L. reuteri showed enhanced amino acid consumption when grown with 0.5 mM GSH. These findings indicate that glutathione is a nutrient for bacterial growth.
谷胱甘肽（GSH）是一种丰富的非蛋白巯基化合物，在细胞内发挥多种作用。以前，我们已经表明唾液乳杆菌具有谷胱甘肽介导的耐酸应答的能力。在本工作中，我们提供了证据表明鼠李糖乳杆菌需要谷胱甘肽，并研究了细胞生长过程中谷胱甘肽的作用。以 0.5mM GSH 作为唯一的硫源补充培养基可增强细胞生长，导致葡萄糖消耗增加，并且与不补充时相比，细胞 GSH 和蛋白质含量增加。此外，当用 0.5mM GSH 培养时，鼠李糖乳杆菌显示出增强的氨基酸消耗。这些发现表明谷胱甘肽是细菌生长的营养物质。
