由产生维生素B12的罗伊氏乳杆菌JCM1112在发酵食品中高水平生产叶酸。
High-Level folate production in fermented foods by the B12 producer Lactobacillus reuteri JCM1112.
作者信息
Santos Filipe, Wegkamp Arno, de Vos Willem M, Smid Eddy J, Hugenholtz Jeroen
机构信息
Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.
出版信息
Appl Environ Microbiol. 2008 May;74(10):3291-4. doi: 10.1128/AEM.02719-07. Epub 2008 Mar 14.
Abstract
We observed that Lactobacillus reuteri JCM1112 produces B(12) and folate. However, the folate/B(12) mass ratio found was far below that desired for human consumption ( approximately 170:1). We used metabolic engineering applying genetic and physiological approaches to improve this ratio and developed a generic and natural process that significantly increases folate production.
摘要
我们观察到罗伊氏乳杆菌JCM1112能产生维生素B12和叶酸。然而，所发现的叶酸与维生素B12的质量比远低于人类食用所需的比例（约为170:1）。我们采用代谢工程，运用遗传和生理学方法来提高这一比例，并开发出一种通用的天然方法，可显著提高叶酸产量。
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