Rosengren S, Arfors K E
Pharmacia Experimental Medicine, La Jolla, California 92037.
Inflammation. 1991 Jun;15(3):159-72. doi: 10.1007/BF00918643.
The microvascular response to two polycationic proteins, poly-L-lysine (mol wt 104,000) and leukocyte elastase, was studied in the hamster cheek pouch microcirculation model. A 2-min topical application of polylysine (100 micrograms/ml) induced vigorous macromolecular leakage from venules only that declined within 30 min. A second application induced significantly less leakage. The leakage was inhibited by admixing polylysine with dextran sulfate prior to application or by giving hamsters an intravenous injection of dextran sulfate. The histamine antagonist pyrilamine did not interfere with the leakage, and only a few degranulated mast cells were found after polylysine application. No intravascular adhesion of leukocytes could be detected. Elastase (100 micrograms/ml) was deposited adjacent to venules with micropipets. The resulting leakage response was not inhibited by L658,758, an inhibitor of elastase enzymatic activity, but by dextran sulfate. These results may prove significant in light of the numerous polycationic proteins present within neutrophil granules.
在仓鼠颊囊微循环模型中研究了两种聚阳离子蛋白，即聚-L-赖氨酸（分子量104,000）和白细胞弹性蛋白酶的微血管反应。局部应用聚赖氨酸（100微克/毫升）2分钟仅诱导小静脉出现强烈的大分子渗漏，这种渗漏在30分钟内下降。再次应用诱导的渗漏明显减少。在应用前将聚赖氨酸与硫酸葡聚糖混合或给仓鼠静脉注射硫酸葡聚糖可抑制渗漏。组胺拮抗剂吡苄明不干扰渗漏，应用聚赖氨酸后仅发现少数脱颗粒的肥大细胞。未检测到白细胞的血管内黏附。用微量移液器将弹性蛋白酶（100微克/毫升）沉积在小静脉附近。弹性蛋白酶酶活性抑制剂L658,758对由此产生的渗漏反应无抑制作用，但硫酸葡聚糖可抑制。鉴于中性粒细胞颗粒中存在众多聚阳离子蛋白，这些结果可能具有重要意义。
