Björk J, Hedqvist P, Arfors K E
Inflammation. 1982 Jun;6(2):189-200. doi: 10.1007/BF00916243.
Leukotriene B4 (LTB4), a metabolite of arachidonic acid, is known to be a potent chemotactic and chemokinetic substance. We have used the hamster cheek pouch microcirculation model to study the effect of LTB4 on vascular permeability and the involvement of neutrophil granulocytes in this response. Intravascular fluorescein-labeled dextran (mol wt 150,000) was used as a tracer of macromolecular permeability. Topical application of LTB4 (150 nM-5 microM) to the hamster cheek pouch resulted in an immediate increase in adhering leukocytes in postcapillary venules and later venules. Leukocyte accumulation was reversible, but continued longer the higher the dose of LTB4 used. Subsequently, a dose-dependent increase in vascular permeability was seen at post-capillary and larger venules, with a maximum 10-20 min after application; the maximum occurred later the higher the dose of LTB4. Depletion of neutrophil granulocytes by pretreatment of the animals with antineutrophil serum obtained from immunized rabbits significantly decreased the permeability response to LTB4, whereas the response to histamine was unaffected. These results suggest that neutrophil granulocytes play a role in LTB4-mediated permeability increase. LTB4 may be of importance both for the leukocyte accumulation and for the edema formation seen in inflammatory reactions.
白三烯B4（LTB4）是花生四烯酸的一种代谢产物，已知是一种强效的趋化和促动物质。我们利用仓鼠颊囊微循环模型来研究LTB4对血管通透性的影响以及中性粒细胞在该反应中的作用。血管内注射荧光素标记的右旋糖酐（分子量150,000）作为大分子通透性的示踪剂。将LTB4（150 nM - 5 microM）局部应用于仓鼠颊囊，导致毛细血管后微静脉和较后微静脉中黏附白细胞立即增加。白细胞聚集是可逆的，但所用LTB4剂量越高，持续时间越长。随后，在毛细血管后微静脉和较大微静脉处可见血管通透性呈剂量依赖性增加，给药后10 - 20分钟达到最大值；LTB4剂量越高，最大值出现得越晚。用从免疫兔获得的抗中性粒细胞血清对动物进行预处理，使中性粒细胞耗竭，显著降低了对LTB4的通透性反应，而对组胺的反应未受影响。这些结果表明中性粒细胞在LTB4介导的通透性增加中起作用。LTB4可能在炎症反应中白细胞聚集和水肿形成方面都具有重要意义。
