与动物转录起始位点相关的微小RNA。
Tiny RNAs associated with transcription start sites in animals.
作者信息
Taft Ryan J, Glazov Evgeny A, Cloonan Nicole, Simons Cas, Stephen Stuart, Faulkner Geoffrey J, Lassmann Timo, Forrest Alistair R R, Grimmond Sean M, Schroder Kate, Irvine Katharine, Arakawa Takahiro, Nakamura Mari, Kubosaki Atsutaka, Hayashida Kengo, Kawazu Chika, Murata Mitsuyoshi, Nishiyori Hiromi, Fukuda Shiro, Kawai Jun, Daub Carsten O, Hume David A, Suzuki Harukazu, Orlando Valerio, Carninci Piero, Hayashizaki Yoshihide, Mattick John S
机构信息
Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Australia.
出版信息
Nat Genet. 2009 May;41(5):572-8. doi: 10.1038/ng.312. Epub 2009 Apr 19.
It has been reported that relatively short RNAs of heterogeneous sizes are derived from sequences near the promoters of eukaryotic genes. In conjunction with the FANTOM4 project, we have identified tiny RNAs with a modal length of 18 nt that map within -60 to +120 nt of transcription start sites (TSSs) in human, chicken and Drosophila. These transcription initiation RNAs (tiRNAs) are derived from sequences on the same strand as the TSS and are preferentially associated with G+C-rich promoters. The 5' ends of tiRNAs show peak density 10-30 nt downstream of TSSs, indicating that they are processed. tiRNAs are generally, although not exclusively, associated with highly expressed transcripts and sites of RNA polymerase II binding. We suggest that tiRNAs may be a general feature of transcription in metazoa and possibly all eukaryotes.
据报道，大小各异的相对较短的RNA源自真核基因启动子附近的序列。结合FANTOM4项目，我们在人类、鸡和果蝇中鉴定出了模式长度为18 nt的微小RNA，它们定位于转录起始位点（TSS）的-60至+120 nt范围内。这些转录起始RNA（tiRNA）源自与TSS同一条链上的序列，并且优先与富含G+C的启动子相关联。tiRNA的5'端在TSS下游10-30 nt处显示出峰值密度，表明它们是经过加工的。tiRNA通常（尽管并非唯一）与高表达转录本以及RNA聚合酶II结合位点相关联。我们认为tiRNA可能是后生动物乃至所有真核生物转录的一个普遍特征。
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