Hu W N, Band R N, Kopachik W J
Department of Zoology, Michigan State University, East Lansing 48824.
Infect Immun. 1991 Nov;59(11):4278-82. doi: 10.1128/iai.59.11.4278-4282.1991.
Protein synthesis patterns of the low-virulence Naegleria fowleri LEE strain from axenic culture, the same strain after mouse brain passage to increase virulence, and the same strain after growth on bacteria were studied. Comparisons of accumulated proteins, in vivo-synthesized proteins, and in vitro-synthesized proteins translated from poly(A)+ mRNA were made. Differences between amoebae from the different treatments were noted. After 6 months in axenic culture, pathogenic protein synthesis patterns were lost and there was a decrease in virulence. Therefore, the increase in virulence is correlated with numerous specific changes in protein synthesis.
研究了来自无菌培养的低毒力福氏耐格里阿米巴LEE菌株、经小鼠脑传代以增加毒力后的同一菌株以及在细菌上生长后的同一菌株的蛋白质合成模式。对积累的蛋白质、体内合成的蛋白质以及从聚腺苷酸加尾mRNA翻译的体外合成蛋白质进行了比较。记录了不同处理的变形虫之间的差异。在无菌培养6个月后，致病蛋白质合成模式丧失，毒力下降。因此，毒力的增加与蛋白质合成中的许多特定变化相关。
