Mattoon Dawn R, Schweitzer Barry
Invitrogen Corporation, Protein Array Center, 688 East Main Street, Branford, CT 06405, USA.
Methods Mol Biol. 2009;524:213-23. doi: 10.1007/978-1-59745-450-6_15.
Antibodies represent the end product of an exquisitely complex biological process including recombination, somatic hypermutation, affinity maturation, and self-tolerance, culminating in binding reagents directed against a vast repertoire of antigens. The resultant high affinity and diversity of specificity of these biomolecules has been exploited through the development of immunoassays and biotherapeutics that inaugurated a new era in experimental molecular biology and pharmaceutical drug development. Despite the utility of antibodies for research applications and in disease treatment, they must be employed in the context of an accurate understanding of their binding profile. High-content microarrays comprised of thousands of native, full length human proteins are an important tool in the assessment of antibody specificity.
抗体是一个极其复杂的生物过程的最终产物，该过程包括重组、体细胞超突变、亲和力成熟和自身耐受，最终产生针对大量抗原的结合试剂。通过免疫测定和生物治疗的发展，这些生物分子所产生的高亲和力和特异性多样性得到了应用，开创了实验分子生物学和药物开发的新纪元。尽管抗体在研究应用和疾病治疗中很有用，但必须在准确了解其结合谱的背景下使用。由数千种天然全长人类蛋白质组成的高内涵微阵列是评估抗体特异性的重要工具。
