Mosing Renee K, Bowser Michael T
University of Minnesota, Minneapolis, MN, USA.
Methods Mol Biol. 2009;535:33-43. doi: 10.1007/978-1-59745-557-2_3.
SELEX (systematic evolution of ligands by exponential enrichment) is a process for isolating DNA or RNA sequences with high affinity and selectivity for molecular targets from random sequence libraries. These sequences are commonly referred to as aptamers. The process typically requires 10-15 cycles of enrichment, PCR amplification and nucleic acid purification to obtain high-affinity aptamers. We have demonstrated that using capillary electrophoresis (CE) as an enrichment step greatly improves the efficiency of the process. CE-SELEX is capable of isolating high-affinity aptamers in as little as 2-4 rounds of selection, shortening the process time from several weeks to as little as a few days.
指数富集配体系统进化技术（SELEX）是一种从随机序列文库中分离对分子靶标具有高亲和力和选择性的DNA或RNA序列的方法。这些序列通常被称为适配体。该过程通常需要10至15轮的富集、PCR扩增和核酸纯化，以获得高亲和力适配体。我们已经证明，将毛细管电泳（CE）用作富集步骤可大大提高该过程的效率。CE-SELEX能够在仅2至4轮筛选中分离出高亲和力适配体，将过程时间从几周缩短至短短几天。
