Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250358, China.
Shandong Provincial Key Laboratory of Animal Resistance Biology, Shandong Normal University, Jinan 250014, China.
Biosensors (Basel). 2022 Dec 27;13(1):39. doi: 10.3390/bios13010039.
An aptamer is a synthetic oligonucleotide, referring to a single-stranded deoxyribonucleic acid or ribonucleic acid ligand produced by synthesis from outside the body using systematic evolution of ligands by exponential enrichment (SELEX) technology. Owing to their special screening process and adjustable tertiary structures, aptamers can bind to multiple targets (small molecules, proteins, and even whole cells) with high specificity and affinity. Moreover, due to their simple preparation and stable modification, they have been widely used to construct biosensors for target detection. The paper-based sensor is a product with a low price, short detection time, simple operation, and other superior characteristics, and is widely used as a rapid detection method. This review mainly focuses on the screening methods of aptamers, paper-based devices, and applicable sensing strategies. Furthermore, the design of the aptamer-based lateral flow assay (LFA), which underlies the most promising devices for commercialization, is emphasized. In addition, the development prospects and potential applications of paper-based biosensors using aptamers as recognition molecules are also discussed.
适体是一种人工合成的寡核苷酸，是指通过体外使用指数富集配体系统进化（SELEX）技术合成的单链脱氧核糖核酸或核糖核酸配体。由于其特殊的筛选过程和可调的三级结构，适体可以与多个目标（小分子、蛋白质，甚至整个细胞）具有高特异性和亲和力。此外，由于其简单的制备和稳定的修饰，它们已被广泛用于构建用于目标检测的生物传感器。纸基传感器是一种具有价格低廉、检测时间短、操作简单等优点的产品，已广泛用作快速检测方法。本综述主要集中于适体的筛选方法、纸基器件和适用的传感策略。此外，还强调了基于适体的侧向流动分析（LFA）的设计，这是最有前途的商业化设备的基础。此外，还讨论了以适体作为识别分子的纸基生物传感器的发展前景和潜在应用。
