利用mRNA展示技术对蛋白质和肽库进行体外筛选。
In vitro selection of protein and peptide libraries using mRNA display.
作者信息
Takahashi Terry T, Roberts Richard W
机构信息
Department of Chemistry, University of Southern California, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2009;535:293-314. doi: 10.1007/978-1-59745-557-2_17.
PMID:19377989
Abstract
In vitro genetic approaches are powerful solutions to the protein design problem. mRNA display is an in vitro selection technique enabling the design of peptide and protein ligands ranging from one to over 100 amino acids. Libraries containing more than 10 trillion unique sequences can be synthesized and sieved with exquisite control over binding stringency and specificity.
摘要
体外遗传方法是解决蛋白质设计问题的有效方法。mRNA展示是一种体外筛选技术，可用于设计长度从一个氨基酸到超过100个氨基酸的肽和蛋白质配体。可以合成包含超过10万亿个独特序列的文库，并在对结合严格性和特异性进行精确控制的情况下进行筛选。
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