Taki Takao, Gonzalez Tania Valdes, Goto-Inoue Naoko, Hayasaka Takahiro, Setou Mitsutoshi
Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho Tokushima, 771-0192, Japan.
Methods Mol Biol. 2009;536:545-56. doi: 10.1007/978-1-59745-542-8_55.
A simple method for transfer of lipids including phospholipids, glycolipids, and neutral lipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane, called TLC blot (far-eastern blot), is presented. Lipids separated on a HPTLC plate are blotted quantitatively. This procedure made it possible to purify individual lipids from a blotted membrane in a short time. Binding study, immunodetection, and mass spectrometric analysis are available for PVDF membrane. Furthermore, the world of molecular species imaging is opened by a scanning analysis with a combination of TLC blot and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometer (TLC-Blot/MALDI-TOF MS).
本文介绍了一种将包括磷脂、糖脂和中性脂质在内的脂质从高效薄层层析（HPTLC）板转移到聚偏二氟乙烯（PVDF）膜上的简单方法，即TLC印迹法（远东印迹法）。在HPTLC板上分离的脂质可被定量印迹。该方法使得在短时间内从印迹膜中纯化单个脂质成为可能。结合研究、免疫检测和质谱分析可用于PVDF膜。此外，通过TLC印迹法与基质辅助激光解吸/电离四极杆离子阱飞行时间质谱仪（TLC-Blot/MALDI-TOF MS）相结合的扫描分析，开启了分子种类成像的领域。
