Pisharath Harshan, Parsons Michael J
Department of Surgery, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
Methods Mol Biol. 2009;546:133-43. doi: 10.1007/978-1-60327-977-2_9.
Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.
前体药物依赖性细胞消融是一种允许进行诱导性和空间限制性细胞破坏的方法。我们描述了在斑马鱼中以组织限制性方式表达大肠杆菌nfsB的转基因方法。该细菌基因编码一种硝基还原酶（NTR），它可使甲硝唑（Met）等前体药物具有细胞毒性。利用与荧光蛋白融合的NTR的表达，人们可以同时使细胞易于接受前体药物处理，并在细胞消融发生时对其进行可视化观察。
