利用绿色荧光融合蛋白监测单纯疱疹病毒复制

The use of green fluorescent fusion proteins to monitor herpes simplex virus replication.

作者信息

Taylor Travis J, Knipe David M

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA, USA.

出版信息

Methods Mol Biol. 2009;515:239-48. doi: 10.1007/978-1-59745-559-6_16.

DOI:10.1007/978-1-59745-559-6_16

PMID:19378129

Abstract

The localization pattern of the seven herpes simplex virus (HSV) DNA replication proteins is dependent upon the status of viral DNA synthesis in the infected cell. Normally, the replication proteins accumulate within replication compartments, which expand as viral DNA synthesis increases. If viral replication is blocked, either by the addition of drugs or a genetic lesion, prereplicative sites are observed. Observing the distribution of a GFP-tagged HSV replication protein can monitor the progression of viral replication. Here, we demonstrate the use of an ICP8-GFP fusion protein to observe the status of HSV replication in cultured cells by the formation of viral replication compartments.

摘要

七种单纯疱疹病毒（HSV）DNA复制蛋白的定位模式取决于受感染细胞中病毒DNA合成的状态。正常情况下，复制蛋白在复制区室中积累，随着病毒DNA合成增加，这些区室会扩大。如果通过添加药物或基因损伤来阻断病毒复制，就会观察到复制前位点。观察绿色荧光蛋白（GFP）标记的HSV复制蛋白的分布可以监测病毒复制的进程。在这里，我们展示了使用ICP8-GFP融合蛋白通过病毒复制区室的形成来观察培养细胞中HSV复制的状态。