Gordon David, Scolding Neil J
University of Bristol Institute of Clinical Neurosciences, Frenchay Hospital, Bristol, UK.
Methods Mol Biol. 2009;549:103-18. doi: 10.1007/978-1-60327-931-4_8.
Mesenchymal stem cells (MSCs) have the potential to play a role in autologous repair of central nervous system injury or disease, circumventing both the complications associated with immune rejection of allogenic cells, and many of the ethical concerns associated with embryonic stem cell use. Human bone marrow-derived MSCs can be extracted relatively simply from the marrow of adult patients and maintained and expanded in culture. More importantly, it has been previously demonstrated that MSCs have the capacity to differentiate into neurons and glia in vitro when grown under appropriate conditions. Multipotent MSCs have also been successfully used in transplantation studies in animal models of disease as diverse as demyelination, stroke, trauma and Parkinson's disease. MSCs therefore provide an attractive and practical source of stem cells for reparative therapy in patients, and in this paper we describe methods for the reproducible culture and neural differentiation of human MSCs generated from patient marrow.
间充质干细胞（MSCs）有潜力在中枢神经系统损伤或疾病的自体修复中发挥作用，既能规避与同种异体细胞免疫排斥相关的并发症，又能避免许多与使用胚胎干细胞相关的伦理问题。人骨髓来源的间充质干细胞可以相对简单地从成年患者的骨髓中提取，并在培养中维持和扩增。更重要的是，先前已经证明，间充质干细胞在适当条件下培养时，具有在体外分化为神经元和神经胶质细胞的能力。多能间充质干细胞也已成功应用于多种疾病动物模型的移植研究，如脱髓鞘疾病、中风、创伤和帕金森病。因此，间充质干细胞为患者的修复治疗提供了一种有吸引力且实用的干细胞来源，在本文中，我们描述了从患者骨髓中生成的人骨髓间充质干细胞的可重复培养和神经分化方法。
