Hamid Q, Azzawi M, Ying S, Moqbel R, Wardlaw A J, Corrigan C J, Bradley B, Durham S R, Collins J V, Jeffery P K
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, UK.
Int Arch Allergy Appl Immunol. 1991;94(1-4):169-70. doi: 10.1159/000235353.
Using in situ hybridization, we have investigated the expression of interleukin-5 (IL-5) mRNA in bronchial biopsies from asthmatics (n = 10) and controls (n = 9). The number of IL-5-nRNA-positive cells were compared with the number of CD25+ and EG2+ cells and total eosinophil counts. Specific hybridization signals for IL-5 mRNA were demonstrated in 6 out of the 10 asthmatic subjects but in none of the controls. The 6 IL-5-mRNA-positive asthmatics tended to have more severe disease and showed a significant increase in the degree of infiltration of the bronchial mucosa by activated T lymphocytes and eosinophils.
我们采用原位杂交技术，研究了哮喘患者（n = 10）和对照组（n = 9）支气管活检组织中白细胞介素-5（IL-5）mRNA的表达情况。将IL-5 mRNA阳性细胞数量与CD25 +和EG2 +细胞数量以及嗜酸性粒细胞总数进行了比较。在10名哮喘患者中有6名检测到IL-5 mRNA的特异性杂交信号，而对照组中未检测到。6名IL-5 mRNA阳性的哮喘患者病情往往更严重，且其支气管黏膜中活化T淋巴细胞和嗜酸性粒细胞的浸润程度显著增加。
