Numao T, Fukuda T, Akutsu I, Nakajima H, Makino S, Sato Y
Department of Internal Medicine and Clinical Immunology, Dokkyo University School of Medicine.
Nihon Kyobu Shikkan Gakkai Zasshi. 1993 Dec;31 Suppl:132-8.
To determine whether IL-5 is locally produced in the bronchial mucosa of asthmatics, and to ascertain the precise cell type producing this cytokine, we examined bronchial biopsies by non-radioactive in situ hybridization and immunohistochemistry. IL-5 cDNA probes were labeled with digoxigenin-dUTP and hybridized to frozen or paraffin tissue sections. Hybridization signals were visualized by an immunohistochemistry technique. An IL-5-producing T cell clone derived from a patient with adult T cell leukemia was used as a positive control. Specific hybridization signals for IL-5 mRNA were observed in the bronchial mucosa of symptomatic asthmatics. No hybridization signal was detected in the control subject in whom no underlying disease was found. Immunohistochemical staining of serial sections using a panel of monoclonal antibodies directed against lymphocyte subsets strongly suggested that cells expressing mRAN for IL-5 were T cells. These results suggest that a cell-cell interaction between T cells and eosinophils through IL-5 may play an important role in the airway inflammation in asthma.
为了确定白细胞介素-5（IL-5）是否在哮喘患者的支气管黏膜中局部产生，并确定产生这种细胞因子的精确细胞类型，我们通过非放射性原位杂交和免疫组织化学检查了支气管活检组织。IL-5 cDNA探针用洋地黄毒苷-dUTP标记，并与冷冻或石蜡组织切片杂交。杂交信号通过免疫组织化学技术可视化。从一名成人T细胞白血病患者获得的产生IL-5的T细胞克隆用作阳性对照。在有症状的哮喘患者的支气管黏膜中观察到IL-5 mRNA的特异性杂交信号。在未发现潜在疾病的对照受试者中未检测到杂交信号。使用一组针对淋巴细胞亚群的单克隆抗体对连续切片进行免疫组织化学染色，强烈提示表达IL-5 mRNA的细胞是T细胞。这些结果表明，T细胞和嗜酸性粒细胞之间通过IL-5的细胞间相互作用可能在哮喘气道炎症中起重要作用。
