Spitzauer S, Rumpold H, Ebner C, Schweiger C, Valenta R, Gabl F, Anrather J, Breitenbach M, Scheiner O, Kraft D
Institute of Clinical Chemistry and Laboratory Diagnostics, University of Vienna, Austria.
Int Arch Allergy Appl Immunol. 1991;94(1-4):346-8. doi: 10.1159/000235399.
The sera from 25 patients with clinical type I allergy against dogs were investigated by means of immunoblotting, using extracts of dog hair/dander, skin, hair, saliva, salivary gland, serum and liver. 96% of the patients' sera showed IgE antibodies reactive with 19- and 23-kilodalton (kDa) proteins in the hair/dander extract. The 23-kDa IgE-binding protein was preferentially detected in the hair extract and saliva but not in skin, salivary gland, serum and liver extracts. The 19-kDa band was strongly expressed in skin, but not in hair, serum and liver. Inhibition experiments using the 23-kDa containing extract prepared from hair and the 19-kDa containing extract prepared from skin revealed that these two proteins are likely to be immunologically independent allergens.
采用狗毛/皮屑、皮肤、毛发、唾液、唾液腺、血清和肝脏的提取物，通过免疫印迹法对25例临床I型犬类过敏患者的血清进行了研究。96%的患者血清显示出与毛发/皮屑提取物中19千道尔顿（kDa）和23千道尔顿蛋白发生反应的IgE抗体。23-kDa IgE结合蛋白在毛发提取物和唾液中优先检测到，但在皮肤、唾液腺、血清和肝脏提取物中未检测到。19-kDa条带在皮肤中强烈表达，但在毛发、血清和肝脏中未表达。使用从毛发制备的含23-kDa提取物和从皮肤制备的含19-kDa提取物进行的抑制实验表明，这两种蛋白可能是免疫独立的过敏原。
Zotero 插件